Trace constituents in whey. Isolation and identification of amines.

Primary and secondary amines were isolated from whey as their 2,4-dinitrophenyl derivatives, separated by thinlayer partition chromatography, and identified by gas chromatography-mass spectrometry. Examination of raw and pasteurized sweet whey, reconstituted spray dried whey powder, and proteinfree ultrafdtration permeate of sweet and acid wheys revealed the same qualitative distribution of amines. Amines identified were methyl-, ethyl-, isobutyl-, dimethyl-, isopropyl-, and n-amylamine, pyrrolidine, and piperidine.